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Molasses as a medium can be use in production of DNA vaccine

Molasses, L.B medium, E.coli DH5α strains, Sucrose, Glucose,K2HPO4,Urea..

1. Introduction

The need of comparison yields was made due to keeping in mind that the final product so obtained from the L.B Medium is costly, by using molasses. The cost of the product can be reduced as molasses medium is three time cheaper then L.B. medium. So in this project the efforts are made to use.

The advantages of the using molasses as an alternative to L.B Medium is apart from the cost effectiveness of the product, molasses as medium is manipulative to requisite micro-organism, because molasses contain the nitrogen in low amount and can be optimize by adding the cheapest source of the growth material. The naturally occurring source as like waste product molasses as substitute from the industry or from the natural plant.

To Fulfill the demand of the growth components and other trace materials for achieving a optimum recombinant plasmid yields so in this project the affords as being made to optimize the molasses to commonly used L.B. medium. Molasses is made from young green sugar cane and is treated with sulfur dioxide, which acts as a preservative, during the sugar extraction process. Unsulfured molasses is made from mature sugar cane and does not require treatment with sulfur during the extraction process.
To make molasses, which is pure sugar cane juice, the sugar cane plant is harvested and stripped of its leaves. Its juice is extracted from the canes, usually by crushing or mashing. The juice is boiled to concentrate and promote the crystallization of the sugar. The majority of sucrose from the original juice has been crystallized but black strap molasses is still mostly sugar by calories (WHF).

Grow the E. coli in molasses as a medium and also in Luria Bertani (L.B) medium. Escherichia coli (E. coli) are a bacterium that is commonly found in the lower intestine of warm-blooded animals. Most E. coli strains are harmless, but some, such as serotype O157:H7, can cause serious food poisoning in humans, and are occasionally responsible for costly product recalls ( Dippold et al., 2005). The harmless strains are part of the normal flora of the gut, and can benefit their hosts by producing vitamin K2, or by preventing the establishment of pathogenic bacteria within the intestine (Hudault et al., Sep.2001). E. coli is Gram-negative, facultative anaerobic and non-sporulating. It can live on a wide variety of substrates. E. coli uses mixed-acid fermentation in anaerobic conditions, producing lactate, succinate, ethanol, acetate and carbon dioxide. Since many pathways in mixed-acid fermentation produce hydrogen gas, these pathways require the levels of hydrogen to be low, as is the case when E. coli lives together with hydrogen-consuming organisms such as methanogens or sulfate-reducing bacteria (Martinko et al., 2006).
Published: 2009-08-03
Author: Nitin Sharma •Arti Lakhtakia

About the author or the publisher
Molasses as a medium can be use in production of DNA vaccine
Nitin Sharma •Arti Lakhtakia
Abstract
Grow the recombinant E. coli for the plasmid DNA using molasses medium as an alternative to L.B media to make the vaccine or protein cost effective. During the initial studies I have found that the molasses may be a good alternative to costly L.B media where one can manipulate the C/N ratio as per requirement of cell. Molasses is one of the best alternatives as it is cheap and can be easily manipulated. Escherichia coli is a bacterium that is commonly found in the lower intestine of warm-blooded animals. it is possible to produce vaccine by molasses medium. The paper is opening a new face of study.


Keywords

Molasses, L.B medium, E.coli DH5α strains, Sucrose, Glucose,K2HPO4,Urea..

Source: nitinsharma.com@gmail.com



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